Course Overview

This course aims to teach the fundamentals of biological mass spectrometry with particular focus on applications to proteomics and analysis of protein modifications. The course will begin with an introduction to the general aspects of mass spectrometry-based peptide sequencing and protein identification, along with recent technological developments in the context of advancing quantitative shotgun proteomic analysis. Current perspectives in global versus directed analysis, speed versus depth in proteomic coverage, will be discussed. This will then be followed by introducing the practical aspects of MS analysis of select protein modifications, at both the levels of single targeted protein of interest and the proteomic scale. Topics include phosphoproteomics; lysine modifications such as acetylation, methylation, ubiquitination, and SUMOylation; cysteine modifications such as oxidation, glutathionylation and S-nitrosylation; and glycosylation. The last further accompanied by an overview of MS-based glycomics and glycoproteomics.

Learning Achievement

Competence

Course prerequisites

Opens to life science Master and PhD Students, as well as keen senior undergraduates major in Biochemistry, who have a basic knowledge of protein chemistry and structures, and the commonly found modifications. No absolute prerequisite is set but will be advantageous and encouraged to have first taken one or more of the core courses offered at 生化所 in the first semester eg 蛋白質修飾機制與細胞訊息；結構生化學. Chemistry graduates interested in biology and major in chemical biology are also encouraged and accepted.

Grading Philosophy

Course schedule

Course type

Online Course Requirement

Instructor

Kay Hooi Khoo

Other information

(College of Life Science) Institute of Biochemical Science,
(College of Medicine) Graduate Institute of Medical Genomics and Proteomics

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