Course Overview

This course aims to teach techniques dealing with recombinant DNA and enable the students to perform a number of basic methodologies frequently used in laboratories which research activities in gene expression, molecular cloning and manipulation of DNA and RNA in general. Another goal is to complement informations given in other regular lessons and transmit a global vision to the student.

Learning Achievement

The course is supposed to give postgrad students the opprotunity to acquire knowledge about Plasmodium and apply moleculares techniques. Further, by applying practical techniques, they learn different aspects about Plasmodium biology and malaria disease. In addition, the students Will have the opportunity to present general topics related to malaria in a series of seminars held in English or Portuguese language.

Competence

Course prerequisites

Grading Philosophy

Written exam and seminar presentations.

Course schedule

1) Cloning of different genes encoding antigens of Plasmodium falciparum; 2) Purification of recombinant proteins by affinity chromatography. 3) PCR; 4) SDS-PAGE; 5) Western blotting; 6) ELISA 7) Real time PCR 8) DNA edition soiftare (ApE). The complete methods are provided in a manual which is delivered in the beginning of the course.

Course type

Online Course Requirement

Instructor

Gerhard Wunderlich, Carsten Wrenger

Other information

Before the course, all selected students meet with the professor in order to organize working groups of two students and distribute seminar themes. The course is mainly meant for undergrad students in the last semesters of Biology, Biomedicine or Pharmaceutical Sciences. In case that the offered seats are not occupied by undergrad students, the remaining slots can be distributed among interested postgrad students.

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