Molecular Biology of Plasmodium falciparum: a Practical Course Biologia Molecular de Plasmodium Falciparum _ um Curso Pr_tico University of Sao Paulo
This course aims to teach techniques dealing with recombinant DNA and enable the students to perform a number of basic methodologies frequently used in laboratories which research activities in gene expression, molecular cloning and manipulation of DNA and RNA in general. Another goal is to complement informations given in other regular lessons and transmit a global vision to the student.
The course is supposed to give postgrad students the opprotunity to acquire knowledge about Plasmodium and apply moleculares techniques. Further, by applying practical techniques, they learn different aspects about Plasmodium biology and malaria disease. In addition, the students Will have the opportunity to present general topics related to malaria in a series of seminars held in English or Portuguese language.
Written exam and seminar presentations.
1) Cloning of different genes encoding antigens of Plasmodium falciparum; 2) Purification of recombinant proteins by affinity chromatography. 3) PCR; 4) SDS-PAGE; 5) Western blotting; 6) ELISA 7) Real time PCR 8) DNA edition soiftare (ApE). The complete methods are provided in a manual which is delivered in the beginning of the course.
Online Course Requirement
Gerhard Wunderlich, Carsten Wrenger
Before the course, all selected students meet with the professor in order to organize working groups of two students and distribute seminar themes. The course is mainly meant for undergrad students in the last semesters of Biology, Biomedicine or Pharmaceutical Sciences. In case that the offered seats are not occupied by undergrad students, the remaining slots can be distributed among interested postgrad students.
Site for Inquiry
Please inquire about the courses at the address below.
Email address: https://ww2.icb.usp.br/ing/