Course Overview

OBJECTIVES:
Knowledge and understanding of the main cellular and preclinical
models in translational research in oncology, and of the main
molecular imaging methods, from single cell to preclinical models.
Introduction to numerical analysis of molecular imaging data

Learning Achievement

Competence

Course prerequisites

Grading Philosophy

Course schedule

- Basic notions and techniques in molecular histopathology: flow
cytometry, specialized primary cell cultures, 3D cell cultures, clonal
cell assays, xenograft models. 
- Imaging and histology of patient tumors taught by pathologists.
Analyses of molecular tumor heterogeneity used in hospital routine.
Molecular imaging techniques: enzymatic and fluorescence detections,
multiple detection. FISH technique. 
- High-resolution imaging: confocal, multiphoton, TIRF. 
- Molecular Imaging in vivo. Xenograft imaging in mice.
Bioluminescence. 
- Lab course in continuity with Cancer Cell Biology teaching unit.
Imaging of cell cycle and proliferation markers expression (p21, Ki67)
following a therapeutic treatment in cancer cells in vitro by
immunofluorescence and on tissue-sections or tumor xenografts by
immunohistochemistry. Image processing with Image J software

Course type

- Theoretical lectures - 2 hours  - Tutorial classes - 10 hours  - Laboratory courses - 14 hours

Online Course Requirement

Instructor

Other information

Language of instruction: English

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