Course Overview

OBJECTIVES 
Theoretical and practical training in Molecular and Cell Biology
techniques.

Learning Achievement

Competence

Course prerequisites

Grading Philosophy

Course schedule

- Manipulation of nucleic acids: Techniques of extraction,
purification, detection and quantification (quantitative PCR). Main
enzymatic tools (for cloning, mRNA mapping), chemical
(oligonucleotides) and enzymatic synthesis.
- DNA structural and chemical modifications (methylation,
G-quadruplex) and specific techniques for their detection.
- Cloning and gene transfer strategies (vectors, libraries and
transfer techniques).
- Strategies for the study of gene expression modification: reporter
genes, deletions, RNA interference, conditional alleles,
over-expression, inducible promotors.
- Interactions' study through Biochemical (SELEX, FRET) and Genetic
(suppression, synthetic lethality, complex haploinsufficiency)
approaches.
- Production and purification of recombinant proteins: methods for
production, purification, detection, fusion and tagged proteins
vectorization. Choice of expression systems (vectors, hosts).

Course type

- Theoretical lectures - 34 hours  - Tutorial classes - 16 hours  - Laboratory courses - 40 hours

Online Course Requirement

Instructor

Other information

Language of instruction: English

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