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Course Detail

Degree
Master
Standard Academic Year
Semester 1
Course delivery methods
Subject
Biological sciences
Program
School
College of Science and Technology
Department
Campus
No value
Classroom
Course Offering Year
2022-2023
Course Offering Month
-
Weekday and Period
Capacity
Credits
9 ECTS
Language
English
Course Number
4TBO701U

Technologies in Molecular and Cell Biology University of Bordeaux

Course Overview

OBJECTIVES 
Theoretical and practical training in Molecular and Cell Biology
techniques.

Learning Achievement

Competence

Course prerequisites

Grading Philosophy

Course schedule

- Manipulation of nucleic acids: Techniques of extraction,
purification, detection and quantification (quantitative PCR). Main
enzymatic tools (for cloning, mRNA mapping), chemical
(oligonucleotides) and enzymatic synthesis.
- DNA structural and chemical modifications (methylation,
G-quadruplex) and specific techniques for their detection.
- Cloning and gene transfer strategies (vectors, libraries and
transfer techniques).
- Strategies for the study of gene expression modification: reporter
genes, deletions, RNA interference, conditional alleles,
over-expression, inducible promotors.
- Interactions' study through Biochemical (SELEX, FRET) and Genetic
(suppression, synthetic lethality, complex haploinsufficiency)
approaches.
- Production and purification of recombinant proteins: methods for
production, purification, detection, fusion and tagged proteins
vectorization. Choice of expression systems (vectors, hosts).

Course type

- Theoretical lectures - 34 hours  - Tutorial classes - 16 hours  - Laboratory courses - 40 hours

Online Course Requirement

Instructor

Other information



Language of instruction: English

Site for Inquiry

Please inquire about the courses at the address below.

Contact person: David Cappellen
david.capellen@u-bordeaux.fr