Technologies in Molecular and Cell Biology University of Bordeaux
OBJECTIVES Theoretical and practical training in Molecular and Cell Biologytechniques.
- Manipulation of nucleic acids: Techniques of extraction,purification, detection and quantification (quantitative PCR). Mainenzymatic tools (for cloning, mRNA mapping), chemical(oligonucleotides) and enzymatic synthesis. - DNA structural and chemical modifications (methylation,G-quadruplex) and specific techniques for their detection. - Cloning and gene transfer strategies (vectors, libraries andtransfer techniques). - Strategies for the study of gene expression modification: reportergenes, deletions, RNA interference, conditional alleles,over-expression, inducible promotors. - Interactions' study through Biochemical (SELEX, FRET) and Genetic(suppression, synthetic lethality, complex haploinsufficiency)approaches. - Production and purification of recombinant proteins: methods forproduction, purification, detection, fusion and tagged proteinsvectorization. Choice of expression systems (vectors, hosts).
- Theoretical lectures - 34 hours - Tutorial classes - 16 hours - Laboratory courses - 40 hours
Online Course Requirement
Language of instruction: English
Site for Inquiry
Please inquire about the courses at the address below.
Contact person: David Cappellendavid.email@example.com